Consumables Details

This section includes additional information on the supplied consumables and the library tube strip adapter.

The NovaSeq X Series 10B Flow Cell, NovaSeq X Series 25B Flow Cell, and NovaSeq X Series 1.5B Flow Cell are patterned flow cells encased in plastic cartridges. The flow cell is a glass-based substrate containing billions of nanowells in an ordered arrangement, which increases the number of output reads and sequencing data. Clusters are generated in the nanowells from which sequencing is then performed.

The NovaSeq X Series 10B Flow Cell and NovaSeq X Series 25B Flow Cell have eight lanes for sequencing pooled libraries. The NovaSeq X Series 1.5B Flow Cell has two lanes for sequencing pooled libraries. Each lane is imaged in multiple swaths. The software then divides the image of each swath into smaller portions called tiles. For more information, refer to Real-Time Analysis .

NovaSeq X Series 10B Flow Cell

NovaSeq X Series 25B Flow Cell

NovaSeq X Series 1.5B Flow Cell

A. Flow cell cartridge
B. 8-lane flow cell (10B)

The undersides of the NovaSeq X Series 10B Flow Cell and NovaSeq X Series 25B Flow Cell contain one inlet gasket and eight outlet gaskets. The underside of the NovaSeq X Series 1.5B Flow Cell contains one inlet gasket and two outlet gaskets. Reagents enter the flow cell lanes through the gasket on the inlet end of the flow cell. Reagents are expelled from the lanes through the gaskets at the outlet end.

Avoid touching the gaskets when handling the flow cell.

The sequencing reagent cartridge is prefilled with reagents, buffers, and wash solution.

The cartridge contains all reagents for a run. The library tube strip and lyo insert are loaded into the thawed cartridge, which is then loaded onto the instrument. After the run begins, reagents and library are automatically transferred from the cartridge to the flow cell. When transporting, carry only one cartridge at a time and grip the cartridge by the sides.

The position #3 and position #8 wells contain formamide. To facilitate the safe disposal of any unused reagent after the sequencing run, this reservoir is removable. For more information, refer to Recycle Reagent Cartridge.

The library tube strip contains one sample tube for each flow cell lane. Each sample tube is numbered. During run planning, enter the sample tube number as the lane number.

Library Tube Strip (8 Lanes)

A. Library tube strip cap
B. Flow cell lane number
C. Sample tube

Library Tube Strip (2 Lanes)

The lyo insert is prefilled with SBS and prepared ExAmp reagents. During sequencing, the instrument automatically rehydrates the ExAmp reagent and then transfers the mixture to the flow cell.

The buffer cartridge is prefilled with sequencing buffers and weighs up to 2.5 kg (5.5 lb). Indentations on the bottom allow buffer cartridges to be stacked. The buffer cartridge is loaded directly onto the instrument.

Use the library tube strip adapter when transporting, centrifuging, and storing the library tube strip. The adapter includes locations to insert the library tube strip sample tubes and cap. If preparing only one library tube strip for sequencing, make sure to balance the adapter with another filled library tube strip before centrifuging.

The dimensions of the adapter are 8.6 cm (3.4 in) x 12.8 cm (5.0 in) x 3.0 cm (1.2 in).