Denature & Dilute Libraries
Use the following instructions to denature and dilute prepared libraries for sequencing on the NovaSeq X Plus. The 10B Flow Cell and the 1.5B Flow Cell have the same recommendations per lane. However, the 10B Flow Cell has eight lanes and the 1.5B Flow Cell has two lanes.
Recommended Loading Concentrations
The optimal loading concentration depends on the library type and insert size. The following table provides the recommended final loading concentrations.
|
Library Type |
10B and 1.5B Flow Cells Final Loading Concentration (pM) |
25B Flow Cell Final Loading Concentration (pM) |
|---|---|---|
|
Illumina Complete Long Reads |
150 |
|
|
Illumina DNA Prep with Enrichment |
150 |
150 |
|
Illumina DNA PCR-Free |
140 |
150 |
|
Illumina Stranded Total RNA with Ribo-Zero Plus |
150 |
150 |
|
TruSeq DNA PCR-Free |
90 |
100 |
|
TruSeq DNA Nano 350 |
160 |
|
|
TruSeq DNA Nano 550 |
160 |
|
|
PhiX |
140 |
140 |
To obtain the optimal seeding concentration to yield the best %PF, you might need to perform a titration of your library type.
