Integrated Software

The system software suite includes integrated applications that perform sequencing runs and analysis.

• NovaSeq X Series Control Software—Controls instrument operation and provides an interface for configuring the system, setting up a sequencing run, monitoring run statistics as sequencing progresses, and viewing DRAGEN data. You can access the control software on-instrument or through a computer connected to a local network.
• Real-Time Analysis (RTA4)—Performs image analysis and base calling during the run. For more information, refer to Real-Time Analysis .
• Universal Copy Service (UCS)—Copies output files to the output folder throughout a run. If applicable, the service also transfers data to BaseSpace Sequence Hub or Illumina Connected Analytics (ICA).
• Illumina DRAGEN Bio-IT Platform—Performs hardware accelerated secondary analysis for a select menu of applications.

The NovaSeq X Series Control Software is interactive and runs automated background processes. RTA4 and UCS run as background processes only.

In the NovaSeq X Series Control Software, select the instrument icon to open the global navigation menu. Select the Settings icon, and then select About to view the Illumina contact information and the following system information:

• NovaSeq X Series Control Software version
• Computer name
• OS image version
• Instrument serial number
• Total run count

To view all system notifications, select the instrument icon to open the global navigation menu, and then select Notifications. The Notifications screen contains two tabs: Notifications and Errors and warnings history. Select the Errors and warnings history tab to view a list of historic errors and warnings. Select the Notifications tab to view a list of current notifications.

When an error or warning occurs, the NovaSeq X Series Control Software displays an alert during the action.

• Critical system errors require immediate attention to shut down the instrument and contact Illumina Technical Support for assistance.
• Non-critical system errors require action before starting or proceeding with the run. Depending on the error, the NovaSeq X Series Control Software provides the appropriate action to resolve the error.
• Warnings do not require action before starting or proceeding with the run. When a warning occurs, the NovaSeq X Series Control Software provides the appropriate action to resolve the warning.
• Notifications provide information regarding events that are not related to the current action. The number of current notifications displays on the Notifications icon in the global navigation menu. Dismiss notifications or resolve the notification on the Notifications tab.

Minimize or exit the control software to access other applications. For example, to access other applications on the instrument to perform operations outside of the control software (eg, locate the sample sheet in file explorer).

1. In the NovaSeq X Series Control Software, select the instrument icon to open the global navigation menu.
2. Select Settings, and then select Minimize software to minimize the control software or Exit software to close the control software.

If you exit the control software, you need to sign in again when reopening.

3. To maximize or open the control software, select NovaSeq X Series Control Software from the toolbar.