Load Wash Consumables

Use the following instructions to load the maintenance wash consumables onto the instrument.

You can initiate a maintenance wash from the Start screen or the Settings screen.

1. To initiate a maintenance wash from the Start screen, select Start and then select Wash.
2. To initiate a maintenance wash from the Settings screen, do as follows.
a. Select the instrument icon to open the global navigation menu.
b. Select Settings, and then select Wash.
3. On the NovaSeq X Plus, select an instrument side for a single-sided wash or select both sides for a dual-sided wash.

If a maintenance wash is required on one or both sides, the side or sides are selected by default.

4. Proceed to Load Wash Flow Cells.

Perform a maintenance wash with either a used 8-lane sequencing flow cell that is already loaded onto the instrument and has not been removed or a newly loaded wash flow cell. If you are performing a maintenance wash after a critical system error stops a run, use a newly loaded wash flow cell.

If performing a maintenance wash with the used sequencing flow cell from the previous sequencing run, the flow cell is already loaded onto the instrument. Proceed to Load Wash Reagent Cartridge.

To use a newly loaded wash flow cell, proceed as follows.

1. On the Maintenance wash screen, select the Load wash flow cell checkbox to replace the used sequencing flow cell with a wash flow cell.
2. Select Load wash flow cell.

After selecting, the display monitor raises and the flow cell door opens. The flow cell light indicates the instrument side that the wash is being performed on.

3. Inspect the flow cell stage for any contaminants (eg, particulates, lint, or dried reagent). If contaminants are visible, clean the flow cell stage as follows.
a. Wet a polynit heatseal wipe with isopropyl alcohol (70%).
b. Gently clean the applicable surface. Wipe in a lengthwise direction only.

Unless you find contaminants on the manifolds, avoid touching them when wiping the flow cell stage.

c. Repeat steps a and b until surfaces are clear of all contaminants.
d. Dry with a new polynit heatseal wipe or an unused side of the used wipe to avoid contamination.
4. Put on a new pair of powder-free gloves to avoid contaminating the glass surface of the flow cell.
5. With the wash flow cell foil package over a flat surface, peel open the foil from the corner tab.
6. Remove the wash flow cell from the package. Grasp the wash flow cell by the sides to avoid touching the glass or the underside gaskets.
7. Inspect the wash flow cell for any contaminants (eg, particulates, lint, or dried reagent). If contaminants are visible, clean the wash flow cell as follows.
a. Wet a polynit heatseal wipe with isopropyl alcohol (70%).
b. Gently clean the applicable surface. Wipe in a lengthwise direction only.
c. Repeat steps a and b until surfaces are clear of all contaminants.
d. Dry with a new polynit heatseal wipe or an unused side of the used wipe to avoid contamination.
8. Discard the package appropriately.
9. Place the wash flow cell in the flow cell stage so that the top surface face upward.
10. After flow cells are loaded, select Close flow cell door.

The control software displays information from the scanned flow cell after 1 minute.

11. Proceed to Load Wash Reagent Cartridge.

Use the following instructions to load the wash reagent cartridge onto the instrument. You do not need to replace the buffer cartridge to perform a maintenance wash. Make sure to properly clean and store the wash reagent cartridge for reuse. The wash reagent cartridge can also withstand autoclaving as a cleaning method.

1. Rinse the wash reagent cartridge with laboratory-grade water twice, and then dry the underside of the cartridge using a paper towel.

Do not dry inside the cartridge wells. Drying the reagent cartridge wash wells can impact the wash process.

2. Add 380 ml wash solution to the wash solution well on the reagent cartridge.
3. Add 49 ml 0.12% reagent grade NaOCl to bleach well on the wash reagent cartridge.
4. On the Wash screen, select Load wash cartridges.

The instrument doors unlock automatically and the system displays information on loading the wash reagent and buffer cartridges.

5. Remove the used sequencing reagent cartridge. Refer to Recycle Used Consumables for instructions on recycling the reagent cartridges.
6. Load the wash reagent cartridge in the right position so that the Illumina label faces you.
7. Close the reagents drawers, and close the instrument doors.
8. Select Verify wash reagents.
9. Empty the used reagent bottles. Refer to Empty Used Reagent Bottles for more information.
10. After all consumables are loaded, select Start wash.

After starting the wash, the instrument doors automatically lock. After the wash completes, the doors unlock and the Start screen displays.

11. To maintain the wash reagent cartridge for multiple uses, rinse with laboratory-grade water twice and store inverted.