Network Connections

The NovaSeq X and NovaSeq X Plus Sequencing Systems require a dedicated network connection. The systems are not intended to store run data.

An external internet connection is optional. The following operations require an external internet connection:

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Uploading instrument performance data to Illumina

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Remote assistance from Illumina Technical Support

Illumina does not install or provide technical support for network connections. For guidelines on how to install and configure a network connection for Illumina systems, refer to Illumina Instrument Control Computer Security and Networking.

The following additional guidelines are applicable to the NovaSeq X and NovaSeq X Plus Sequencing Systems:

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To connect with local networks, the instrument includes two RJ-45 copper interfaces for use with the cables provided. Direct fiber connections to the instrument are not possible.

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A 10 gigabit (Gb) intranet connection to network storage is required. Lower connection speeds result in reduced instrument availability, increased data transfer times, and might also impact sequencing run performance.

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Switches must be non-blocking with full duplex ports and link speed auto-negotiation turned on.

Use the following recommended network bandwidth per instrument for connections, based on 85–90% network efficiency.

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800 megabits per second (Mb/s) (primary analysis only) or ~3.5 gigabits per second (Gb/s) (primary and secondary analysis) sustained network bandwidth for storing data to local network storage.

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800 Mb/s network bandwidth for uploading primary analysis data to the cloud.

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15 Mb/s network bandwidth for run monitoring or Illumina Proactive Support only.

Primary analysis files include BCL files and ancillary files generated by RTA4. Secondary analysis files include on-instrument DRAGEN output files.