Load Lyo Insert and Library Tube Strip

Before sequencing, load the lyo insert and library tube strip into the reagent cartridge as follows.

1.

Record the library tube ID on the library tube strip. The library tube ID is used when planning a sequencing run.

2.

Uncap the library tube strip. Do not pierce the library tube strip foils.

3.

Dispense the appropriate amount of denatured library or denatured library with PhiX into each sample tube.

•

[25B] 275 µl

•

[10B, 1.5B] 165 µl

4.

Add the appropriate amount of pre-load buffer to any unused sample tubes.

•

[25B] 275 µl

•

[10B, 1.5B] 165 µl

5.

After dispensing libraries, cap the library tube strip.

Make sure that no air gaps are present at the bottom of the tubes.

6.

Centrifuge at 280 × g for 1 minute using the library tube strip adapter.

Make sure to counterbalance the loaded library tube strip adapter with another adapter that is loaded with a library tube strip filled to 275 or 165 µl per tube.

7.

If libraries are not collected in the bottom of the tube, repeat centrifuge.

8.

Insert the library tube strip into the reagent cartridge and press down.

An audible click indicates that the library tube strip is in place.

9.

Insert the lyo insert into the reagent cartridge and press down.

An audible click indicates that the lyo insert is in place.