Prepare Wash Solution and NaOCl
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1.
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Add 400 ml laboratory-grade water to a 500 ml centrifuge bottle. |
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2.
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Add 200 µl 100% Tween 20 or an analogous volume of a diluted tween solution. For example, 1 ml 20% Tween 20 in laboratory-grade water. |
Use a freshly prepared dilution of Tween 20 to limit the introduction of contaminants into the fluidics system.
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4.
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Combine the following volumes in a 50 ml centrifuge tube to prepare 49 ml of 0.12% reagent grade NaOCl: |
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5% reagent grade NaOCl (1.2 ml) |
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Deionized water (48 ml) |
Only use reagent grade NaOCl. Avoid general-purpose bleach products as these can contain ammonia compounds, which may lead to runs with low percentage passing filter reads.
