Requeue Secondary Analysis

During secondary analysis, you can requeue the run to perform on-instrument DRAGEN analysis again. You cannot requeue if there is a sequencing run in-progress or if the run data has been removed from the instrument. For information on how to requeue secondary analysis for a run planned in BaseSpace Sequence Hub, refer to the BaseSpace Sequence Hub support page.

1.

Select the instrument icon to open the global navigation menu.

2.

Select Runs, and then navigate to the Completed tab.

3.

Select the run to requeue.

4.

Under secondary analysis, select Requeue analysis.

5.

If the requeue analysis is for a local run, select one of the following requeue options:

•

Requeue analysis from this run—Perform requeue using the files generated in the selected run. You can select a folder to output the requeued analysis files to.

•

Requeue analysis from a sample sheet—Upload a sample sheet to perform requeue. The requeue uses the analysis configurations specified in the sample sheet.

To requeue a local run with 12 index cycles, you must requeue analysis from a sample sheet.

6.

If the requeue analysis is for a manual run, select Requeue analysis from sample sheet and upload a sample sheet.

7.

[Optional] Select the Skip sample sheet validation checkbox.

To skip validation, the sample sheet must be correctly formatted.

8.

Enter a sequencing data file path.

This path determines the external storage location for the requeue analysis run.

9.

Enter a description for the requeue in the Reason field.

10.

Select Requeue analysis when finished.

11.

Perform any of the following actions:

•

To exclude a configuration from the requeue, select Delete next to the configuration name.

•

To change configuration information, select Edit next to the configuration name.

•

To include additional configurations, select Add configuration.

12.

Select Requeue analysis when finished.