Monitor Run Progress
You can monitor run progress, cancel a run, or start a new run on the Sequencing screen. You can monitor run progress on-instrument or using a networked computer. If you have cloud run monitoring enabled, you can view run progress in BaseSpace Sequence Hub. To view additional run details and run status, refer to Run Management.
To view additional metrics and visualizations, you can use the Sequencing Analysis Viewer (SAV). For more information on SAV, refer to the Sequencing Analysis Viewer support site page.
If the run is canceled at any time, a post-run wash is performed automatically. If a post-run wash fails or is not performed due to error, perform a maintenance wash before beginning a new sequencing run. Refer to Perform a Maintenance Wash for instructions.
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Monitor run status on the Sequencing screen or the Active tab in the Runs screen. |
The Sequencing screen contains the estimated run completion time, which requires 10 previous runs to calculate accurate run completion time.
The Active tab on the Runs screen includes the time the process was started and additional information on the run status. The status indicates which of the following activities are in progress:
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Sequencing data transfer to external storage |
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Secondary analysis data transfer to external storage |
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Monitor the following metrics on the Sequencing or Runs screen. |
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% ≥ Q30—The average percentage of base calls with a Q-score ≥ 30. |
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Yield—The expected number of bases called for the run. |
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Total reads PF—The number of paired-ends (if applicable) reads passing filtering (in millions). |
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To review any additional run details, select the run name on the Sequencing screen or the Active tab in the Runs screen. |
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After the run completes, you can view additional run results by selecting the run name on the Sequencing screen or the Completed tab in the Runs screen. |
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Leave the consumables in the instrument. Do not remove until prompted during your next run setup. |
