Sequencing Consumables

Sequencing on the NovaSeq X or NovaSeq X Plus requires one single-use NovaSeq X Series reagent kit. For dual-sided sequencing runs on the NovaSeq X Plus, use two reagent kits. Each component uses radio-frequency identification (RFID) for accurate consumable tracking and compatibility. The reagent kit contains the following components:

• Buffer cartridge
• Flow cell
• Library tube strip
• Lyo insert
• Pre-load buffer
• Reagent cartridge

NovaSeq X Series consumables are packaged in the following configurations.

Kit Name

Illumina Catalog Number

NovaSeq X Series 25B Reagent Kit

20104706 (300 cycles)

20125968 (200 cycles)

20125967 (100 cycles)

NovaSeq X Series 10B Reagent Kit

 

20085594 (300 cycles)

20085595 (200 cycles)

20085596 (100 cycles)

NovaSeq X Series 1.5B Reagent Kit

20104705 (300 cycles)

20104704 (200 cycles)

20104703 (100 cycles)

When you receive your kit, visually inspect each component and promptly store components at the indicated temperature to ensure proper performance.

All kit components are shipped at room temperature.

Use the following specifications to determine storage requirements. A single flow cell run requires one of each of the following items. A dual flow cell run requires two of each item. When you receive your kit, promptly store components at the indicated temperature to ensure proper performance.

Item

Storage Temperature

Consumable Dimensions

Package Dimensions

Reagent cartridge

-25°C to -15°C

19.9 cm x 29.3 cm x 8.5 cm

(7.8 in x 11.5 in x 3.3 in)

31.2 cm x 23.9 cm x 9.7 cm

(12.3 in x 9.4 in x 3.8 in)

Lyo insert

-25°C to -15°C

9.1 cm x 2.6 cm x 8.3 cm

(3.6 in x 1.0 in x 3.3 in)

14.2 cm x 19.1 cm x 2.5 cm

(5.6 in x 7.5 in x 1.0 in)

Custom primer buffer*

-25°C to -15°C

11.9 cm x 1.8 cm

(4.7 in x 0.7 in)

3.8 cm x 4.9 cm x 12.6 cm

(1.5 in x 1.9 in x 5.0 in)

Pre-load buffer*

-25°C to -15°C

9.2 cm x 1.6 cm

(3.6 in x 0.6 in)

3.8 cm x 3.8 cm x 10.2 cm

(1.5 in x 1.5 in x 4.0 in)

Flow cell

2°C to 8°C

8.2 cm x 10.8 cm x 1.3 cm

(3.2 in x 4.3 in x 0.5 in)

15.2 cm x 2.5 cm x 20.1 cm

(6.0 in x 1 in x 7.9 in)

Buffer cartridge

15°C to 30°C

8.0 cm x 30.9 cm x 13.3 cm

(3.1 in x 12.2 in x 5.2 in)

31.2 cm x 8.4 cm x 14.0 cm

(12.3 in x 3.3 in x 5.5 in)

Library tube strip

15°C to 30°C

9.0 cm x 1.9 cm x 4.1 cm

(3.5 in x 0.7 in x 1.6 in)

9.7 cm x 3.8 cm x 5.1 cm

(3.8 in x 1.5 in x 2 in)

* Store the pre-load and custom primer buffers vertically and in the packaging to prevent leaks.

Avoid dropping cartridges. Injury could occur if dropped. Skin irritation could occur if reagents leak from cartridges. Inspect cartridges for cracks before use.

The reagent cartridge, buffer cartridge, and lyo insert contain reagents that are sensitive to light. Keep the items packaged until use and store in the dark with no sources of light.