Start a Planned Run

If using BaseSpace Sequence Hub or ICA, make sure you have configured your cloud settings. Refer to Configure Cloud Settings and Proactive Support for more information.

1. If you are not signed in, follow the instructions in Sign In and Sign Out.
2. Navigate to the Start screen, and then select Start.
3. Select Sequencing.
4. On the NovaSeq X Plus, select an instrument side for a single-sided run or select both sides for a dual-sided run.
5. Select a run from the list of planned runs.

For a dual-sided run on the NovaSeq X Plus, select a run for each side.

6. Select Review, and then review the run information.
7. To use custom primers, select the appropriate Custom primer checkboxes.

For more information on custom primers, refer to Custom Primers.

8. If the instrument is connected to the cloud, confirm the cloud run setting.
9. Confirm the output folder.

For runs with cloud storage enabled, the following options are available:

To transfer run data to a local storage folder in addition to the cloud, provide an output folder.
To only transfer run data to the cloud, select Don't transfer run data to external output folder.

To change the default output folder, refer to Specify the Default Output Folder Location for more information.

10. If performing local secondary analysis, confirm BCL data folder transfer setting.

To update the default run data management selection, refer to Customize System Settings.

11. [Optional] Select a custom recipe file.
12. After reviewing the run information, select Load consumables.