Thaw Consumables

Use the following instructions to thaw consumables before sequencing.

Use the following instructions to thaw the reagent cartridge in a room temperature water bath (15°C to 30°C).

Inserting the library tube strip or lyo insert into the reagent cartridge while thawing might cause reduced data quality or run failure.

1. Put on a new pair of powder-free gloves and remove the cartridge from -25°C to -15°C storage.
2. Remove the cartridge from the box, and then remove it from the bag.
3. Submerge the reagent cartridge in a room temperature, laboratory-grade water bath until the water reaches the bottom of the cartridge cover.

Using hot water for thawing reagents might cause reduced data quality or run failure.

4. Thaw for 4 hours. Do not exceed 24 hours.
5. [25B, 10B] To confirm reagents are thawed, inspect the position #30 well on the underside of the cartridge and make sure that the contents are free of ice.

For the NovaSeq X Series 1.5B Reagent Kit, Illumina recommends strictly following the provided thaw conditions and times. Visual checks to confirm reagents are thawed might not be accurate for this kit.

6. Thoroughly dry the cartridge using paper towels. Dry between wells beneath the cartridge so that all water is removed.
7. Invert or gently the tap the bottom of the cartridge on the bench to remove excess water.
8. Inspect the foil seals for water.
9. If water is still present, blot dry with a lint-free tissue.
10. Invert the cartridge 10 times to mix reagents.
11. Gently tap the bottom of the cartridge on the bench to reduce air bubbles.
12. If the reagent cartridge cannot be loaded into the instrument within 24 hours, store at 2°C to 8°C for up to 72 hours or return to -25°C to -15°C storage for up to 7 days. After thawing, do not refreeze more than one time.

Use the following instructions to thaw the reagent cartridge in a 2°C to 8°C refrigerator.

Inserting the library tube strip or lyo insert into the reagent cartridge while thawing might cause reduced data quality or run failure.

1. Put on a new pair of powder-free gloves and remove the cartridge from -25°C to -15°C storage.
2. Remove the cartridge from the box, and then remove from the bag.
3. Thaw in a 2°C to 8°C refrigerator for 48 hours.
4. Invert the cartridge 10 times to mix reagents.
5. Gently tap the bottom of the cartridge on the bench to reduce air bubbles.
6. If the reagent cartridge cannot be loaded into the instrument within 24 hours, store at 2°C to 8°C for up to 72 hours or return to -25°C to -15°C storage for up to 7 days. After thawing, do not refreeze more than one time.
1. Remove the lyo insert from -25°C to -15°C storage.
2. Thaw at room temperature for 10 minutes.
3. If lyo insert cannot be loaded within 24 hours, return to -25°C to -15°C storage. After thawing, do not refreeze more than one time.
1. Remove pre-load and custom primer buffers insert from -25°C to -15°C storage.
2. Thaw at room temperature for 10 minutes, and then invert five times.
3. If pre-load and custom primer buffers cannot be loaded within 8 hours, return to -25°C to -15°C storage. After thawing, do not refreeze more than one time.
1. Remove a new flow cell package from 2°C to 8°C storage.
2. Set the sealed flow cell package aside for 10–15 minutes to allow the flow cell to reach room temperature.
3. Leave flow cell in package until use. Use the flow cell within 2 hours of removing it from storage. If flow cell cannot be used within 2 hours, return to 2°C to 8°C storage and use within 24 hours.