Host Network and Control Computer Guidelines

The iSeq 100 Sequencing System is designed for use with a network, regardless of whether runs are configured for BaseSpace Sequence Hub. The following operations require an external internet connection, even if BaseSpace Sequence Hub is not used:

• Automatically updating the control software.
• Uploading instrument performance data to Illumina.
• Configuring the output folder to reside on your network.
• Remote assistance from Illumina Technical Support.

The default network configuration is sufficient to transfer data and otherwise operate the system. If your organization has specific network requirements, consult your IT representative for help with advanced network settings. This section provides network guidelines intended for IT representatives.

Use an ethernet connection to transfer data. Variable network strength can extend data transfer and delay subsequent runs. Until Universal Copy Service (UCS) completes data transfer for the previous run, a new run cannot be started.

BaseSpace Sequence Hub requires up to 900 MB of storage to accommodate data uploaded from a run. If data are stored locally, use the following approximate file sizes as a reference. These files are generated by a sequencing run and subsequent analysis.

Output File Type

Approximate Size

BAM

< 600 MB

BCL

850 MB

FASTQ

850 MB

gVCF, VCF

< 10 MB

InterOp

3 MB

Illumina does not install or provide technical support for network connections. Review network maintenance activities for potential compatibility risks with the iSeq 100.

Use the following guidelines to install and configure a network connection:

• Use a dedicated 1 Gb connection between the instrument and data management system. Make this connection directly or through a network switch.
• The required bandwidth is 5 Mb/s/instrument for internal network uploads, BaseSpace Sequence Hub network uploads, and instrument operational data uploads.
• Switches and other network equipment must have a minimum connection speed of 1 Gb per second. Total use on any switch must not exceed the rated speed.
– Calculate the total capacity of the workload on each network switch. The number of connected instruments and ancillary equipment, such as a printer, can impact capacity.
– If the instrument is operating in a complex network environment, use managed switches. For less complex environments with few devices on the network, managed switches are not necessary.
• Cables must be CAT-5e, or better. The shipping box contains a shielded CAT-5e network cable that is 3 m (9.8 ft) long.
• If possible, isolate sequencing traffic from other network traffic.
• Configuring the iSeq 100 for use with a proxy server depends on the unique setup of your network. For instructions, refer to Connect to a Proxy Server.