Consumables and Equipment
The following user-supplied consumables and equipment are used for sequencing, maintenance, and troubleshooting.
Consumables for Sequencing
|
Supplier |
Purpose |
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|---|---|---|---|---|---|---|---|---|---|---|---|
|
Disposable gloves, powder-free |
General lab supplier |
General purpose. |
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|
iSeq 100 i1 Reagent v2 kits |
Illumina, catalog #:
|
Provides the reagents and flow cell for a run. |
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|
Microtubes, 1.5 ml |
Fisher Scientific, catalog # 14-222-158, or equivalent low-bind tubes |
Diluting libraries to the loading concentration. |
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|
Paper towels |
General lab supplier |
Drying the cartridge after a water bath. |
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|
Pipette tips, 20 µl |
General lab supplier |
Diluting and loading libraries. |
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|
Pipette tips, 100 µl |
General lab supplier |
Diluting and loading libraries. |
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|
Resuspension Buffer (RSB) |
Illumina, provided with library prep kits |
Diluting libraries to the loading concentration. |
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|
[Optional] 10 mM Tris-HCl, pH 8.5 |
General lab supplier |
Substitute for RSB to dilute libraries to the loading concentration. |
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|
[Optional] PhiX Control v3 |
Illumina, catalog # FC-110-3001 |
Performing a PhiX-only run or spiking in a PhiX control. |
Consumables for Maintenance and Troubleshooting
|
Consumable |
Supplier |
Purpose |
|---|---|---|
|
VWR, catalog # 16200-218, or equivalent |
Decontaminating the instrument and cleaning work surfaces. |
|
|
Disposable gloves, powder-free |
General lab supplier |
General purpose. |
|
iSeq 100 Spare Drip Tray Pad¹ |
Illumina, catalog # 20023927 |
Lining the drip tray to absorb any leaked fluids. |
|
iSeq 100 Spare Air Filter¹ |
Illumina, catalog # 20023928 |
Replacing the air filter every six months. |
|
iSeq 100 System test kit² |
Illumina, catalog # 20024141 |
Performing a system check. |
|
Isopropyl alcohol wipes, 70% |
VWR, catalog # 95041-714, or equivalent |
Cleaning the instrument and reusable test flow cell. |
|
Lab tissue, low-lint |
VWR, catalog # 21905-026, or equivalent |
Drying the drip tray and reusable test flow cell. |
|
Paper towels |
General lab supplier |
Drying fluid around the instrument. |
|
[Optional] Bleach solution, 10% |
VWR, catalog # 16003-740 (32 oz), 16003-742 (16 oz), or equivalent |
Cleaning work surfaces after decontamination. |
|
[Optional] Ethanol wipes, 70% |
Fisher Scientific, catalog # 19-037-876, or equivalent |
Substitute for isopropyl alcohol wipes to clean the instrument and reusable test flow cell. |
¹ The instrument ships with one installed and one spare. When not under warranty, replacements are user-supplied. Keep packaged until use.
² Replaces the reusable test components shipped with the instrument when they expire after 5 years or exceed 130 uses.
Equipment
|
Item |
Source |
Purpose |
|---|---|---|
|
Vortex mixer |
General lab supplier |
Mix Reagents |
|
Mini Centrifuge |
General lab supplier |
Spin down reagents |
|
General lab supplier |
Storing the cartridge. |
|
|
Ice bucket |
General lab supplier |
Setting aside libraries. |
|
Pipette, 10 µl |
General lab supplier |
Diluting libraries to the loading concentration. |
|
Pipette, 20 µl |
General lab supplier |
Diluting libraries to the loading concentration. |
|
Pipette, 100 µl |
General lab supplier |
Diluting libraries to the loading concentration. |
|
Refrigerator, 2°C to 8°C |
General lab supplier |
Storing the flow cell. |
|
[Optional] Keyboard |
General lab supplier |
Supplementing the onscreen keyboard. |
|
[Optional] Mouse |
General lab supplier |
Supplementing the touch screen interface. |
|
[Optional] Water bath |
General lab supplier |
Thawing the cartridge. |
