Maintenance

The following sections provide maintenance procedures for the Illumina iSeq 100 Sequencing System.

A sequencing run requires about 2 Gb of local hard drive space. A warning notification displays when space is low. Use the following steps to delete completed runs and clear space.

Only delete runs using the iSeq 100 Control Software rather than manually through the operating system. Deleting runs manually can negatively impact the control software.

1. From the control software menu, select Process Management.

The Process Management screen appears with a list of runs saved to the local hard drive.

2. For the run you want to delete, select Delete Run.

Deleting a run deletes the local run folder. The output folder, which is a copy of the run folder, is retained.

3. In the dialog box, select Yes, Delete Run to confirm deleting the run.
4. Repeat steps 2 and 3 for each run you want to delete.
5. When you are finished, close Process Management to return to the Home screen.

The air filter is a single-use foam piece that covers the two fans on the back of the instrument. It ensures proper cooling and prevents debris from entering the system. The instrument is shipped with one air filter installed and one spare. Additional spares are included with the warranty, or they can be purchased from Illumina.

The software prompts for an air filter change every six months from when first time setup was started. Use the following instructions to replace an expired air filter.

1. Position the instrument so you can easily access the back.
2. On the back of the instrument, press the right side of the top panel to disengage as shown in the following illustration.

3. Remove the panel from the instrument.

4. Remove the foam air filter from the center of the panel and discard.

5. Place a new air filter into the panel, and press to secure.
6. Insert the two panel hooks into the holes in the instrument and press the panel into place.

7. Return the instrument to the original placement.
8. Select Filter Changed to proceed.

Use the following instructions to safely relocate an instrument. Make sure that the new location meets the requirements described in Site Preparation.

If you are returning an instrument, skip this section and refer to Advanced Exchange.

1. From the control software menu, select Shut Down Instrument.
2. If the system does not shut down, hold the power button on the left side of the instrument until the lights fade.
3. When the power button pulses, press the power off (Ο) side of the toggle switch on the rear panel.

The power button might continue to pulse after the power is turned off.

Rear Panel Components

  1. Ethernet port—Optional Ethernet cable connection.
  2. USB ports—Two ports for connecting auxiliary components.
  3. Toggle switch—Turns instrument power on and off.
  4. AC power inlet—Power cord connection.
4. Disconnect the power cord from the wall outlet, and then from the AC power outlet on the rear panel.
5. If applicable, disconnect the Ethernet cable from the wall outlet, and then from the Ethernet port on the rear panel.
6. Relocate the instrument to the desired location.

The instrument weighs 15.9 kg (35 lbs) and requires a two-person lift.

7. Raise the monitor.
8. If the instrument is connected to a network, connect the Ethernet cable to an Ethernet port.
9. Connect the power cord to the AC inlet on the rear panel, and then to a wall outlet.
10. Press the power on (I) side of the toggle switch.
11. When the power button pulses, press it.
12. Wait about 5 minutes for the operating system to load. When the operating system is loaded, log in to the system.

The control software is launched and initializes the system. Wait about 5 minutes for system initialization. The Home screen appears when initialization is complete.

External System Components

  1. Power button—Controls instrument power and indicates whether the system is on (glows), off (dark), or off but with AC power (pulses).
  2. Touch-screen monitor—Enables on-instrument configuration and setup using the iSeq 100 Control Software interface.
  3. Status bar—Indicates system status as ready to sequence (green), processing (blue), or needs attention (orange).
  4. Consumables compartment—Contains consumables during a run.
  5. Drip tray door—Provides access to the drip tray, which catches leaked fluids.