System Overview
The Illumina iSeq 100 Sequencing System provides a targeted approach to next-generation sequencing (NGS). This applications-focused system packages Illumina sequencing technology into a cost-effective benchtop sequencing system.
Features
| • | Accessibility and reliability—The iSeq 100 has a small footprint and is easy to install and use. Fluidics and imaging components are built into the consumable, simplifying instrument maintenance. |
| • | Single-step consumable loading—A single-use cartridge is prefilled with all reagents required for a run. Libraries, custom primers, and a sensor-equipped flow cell load directly into the cartridge, which is then loaded onto the instrument. Integrated identification enables accurate tracking. |
| • | iSeq 100 software—A suite of integrated software controls instrument operations, processes images, and generates base calls. This suite features on-instrument data analysis and data transfer tools for external analysis. |
| – | On-instrument analysis—Local Run Manager inputs sample information and then analyzes run data using the analysis module specified for the run. The software includes a suite of analysis modules. |
| – | Cloud-based analysis—The sequencing workflow is integrated with BaseSpace Sequence Hub, the Illumina cloud computing environment for run monitoring, data analysis, storage, and collaboration. Output files are streamed in real time to BaseSpace Sequence Hub for analysis. |
Sample to Analysis
The following diagram illustrates the complete sequencing workflow, from experimental design to analyzing data. Tools and documentation are included with each step. This guide covers the sequence libraries step. The iSeq 100 Sequencing System support pages on the Illumina support center provide additional resources.
Sample to Analysis Workflow
