Perform A-Tailing

Consumables

•

ATL4 (A-Tailing Mix)

•

Microseal 'B' adhesive seals

Preparation

1.

Prepare the following consumables:

•

ATL4—Vortex to mix, and then centrifuge briefly. Make sure that ATL4 is dissolved completely.

2.

Save the following ATL program on the thermal cycler:

•

Choose the preheat lid option and set to 100°C

•

Set the reaction volume to 90 μl

•

37°C for 20 minutes

•

70°C for 5 minutes

•

4°C for 5 minutes

•

Hold at 4°C

Procedure

1.

Add 35 µl ATL4 to each well of the LP plate.

2.

Apply Microseal 'B' and shake at 1800 rpm for 1 minute.

3.

Centrifuge briefly.

4.

Place on the preprogrammed thermal cycler and run the ATL program.

5.

When the program reaches the 4°C hold, immediately proceed to the next step.