Perform End Repair

Consumables

• ERP6 (End Repair Mix)
• Microseal 'B' adhesive seal

Preparation

1. Prepare the following consumables:
• ERP6—Vortex to mix, and then centrifuge briefly.
2. Save the following ERP program on the thermal cycler:
• Choose the preheat lid option and set to 100°C
• Set the reaction volume to 55 µl
• 30°C for 30 minutes
• 70°C for 10 minutes
• 4°C for 5 minutes
• Hold at 4°C

Procedure

1. Remove the LP plate from ice.
2. Add 10 µl ERP6 to each well of the LP plate.
3. Apply Microseal 'B' and shake at 1800 rpm for 1 minute.
4. Centrifuge briefly.
5. Place on the preprogrammed thermal cycler and run the ERP program.
6. When the program reaches the 4°C hold, immediately proceed to the next step.