Overview

The Illumina 5-Base DNA Prep enables library preparation from high quality genomic DNA (gDNA) or cell-free DNA (cfDNA) input.

Illumina 5-Base DNA Prep:

Uses a proprietary enzymatic reaction to convert methylated cytosines to thymine in a single step, while retaining the identity of unmethylated cytosines.
Generates sequencing-ready libraries from a minimum of 50 ng gDNA or 1 ng cfDNA.
Enables the detection of both methylation and genomic variants from a single library prep using the DRAGEN Germline or DRAGEN Somatic applications available on BaseSpace Sequence Hub.