Protocol
This section describes the Illumina 5-Base DNA Prep protocol and provides instructions for preparing gDNA and cfDNA input. Use the procedures and reagents appropriate for your input type. After completing input preparation, continue the protocol with Prepare Reagents.
Before you begin, do as follows.
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Review the entire protocol. |
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Review the preparation sections appropriate for your input type. |
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Before proceeding, confirm kit contents and make sure that you have the required equipment and consumables. This protocol requires library prep reagents and indexes. For details, refer to Product Contents. |
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Have ice available. The library prep PCR plate and many of the consumables must be placed on ice during the protocol. |
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Follow the protocol in the order shown, using the specified volumes and incubation parameters. |
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Unless a safe stopping point is specified in the protocol, proceed immediately to the next step. |
Illumina 5-Base DNA Prep Kit Workflow
The following diagram illustrates the Illumina 5-Base DNA Prep kit workflow.
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Each protocol section starts with Prepare Reagents and ends at a safe stopping point. |
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Time estimates are based on preparing 24 samples using a multichannel pipette. |
