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MRR (Methylation Resuspension Reagent) |
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UMI3 (Unique Molecular Identifier) |
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Microseal 'B' adhesive seals |
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1.
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Prepare the following consumables: |
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LIGX—Before use, centrifuge briefly, pipette to mix, and then centrifuge briefly again. |
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MRR—Bring to room temperature to prepare for Clean Up Ligation. |
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UMI3—Vortex on medium-high speed for at least 10 seconds to mix, and then centrifuge briefly. |
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2.
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Save the following LIG program on the thermal cycler: |
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Choose the preheat lid option and set to 42°C |
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Set the reaction volume to 100 μl |
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1.
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Add 5 μl LIGX to each well of the LP plate. |
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2.
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Add 5 μl UMI3 adapters to each well. |
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3.
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Apply Microseal 'B' and shake at 1800 rpm for 1 minute. |
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5.
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Place on the preprogrammed thermal cycler and run the LIG program. |
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6.
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When the program reaches the 4°C hold, immediately proceed to the next step. |
