Consumables & Equipment
The protocol requires the following user-supplied consumables and equipment.
Consumables
|
Consumable |
Supplier |
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|---|---|---|---|---|---|---|---|
|
1 M Tris-HCl pH 7.5 |
Teknova, catalog # T5075 |
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|
10% Bovine Serum Albumin (BSA) |
Sigma-Aldrich, catalog # A1595 |
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|
100% Anhydrous DMSO |
Thermo Fisher Scientific, catalog # D12345 |
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|
Absolute methanol (MeOH) |
Sigma-Aldrich, catalog # 34860 |
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|
Cell counting materials (eg, AO/PI) |
General lab supplier |
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|
Cell Suspension Buffer (CSB) |
Included in all Illumina Single Cell Prep kits |
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|
Conical tube, 15 ml, sterile and nuclease-free |
General lab supplier |
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|
DEPC-Treated Water (μl) |
Thermo Fisher Scientific, CAT#AM9906 |
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|
DTT (1 M) |
Sigma-Aldrich, catalog # 646563 |
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|
[Optional] DTT powder, solid |
Sigma-Aldrich, catalog # D9779 or similar |
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|
Low retention microcentrifuge tube, 1.5 ml |
General lab supplier |
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|
Nuclease-free water |
Thermo Fisher, catalog # AM9937 or general lab supplier |
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|
NxGen RNase inhibitor (U/μl) |
Biosearch Technologies, catalog # 30281-1 |
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|
Pierce DSP, No-Weigh Format |
Thermo Fisher Scientific, catalog # A35393 |
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|
Pipette tips, filtered, low retention, sterilized, 20 μl |
One of the following suppliers:
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|
Pipette tips, filtered, low retention, sterilized, 200 μl |
One of the following suppliers:
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Pipette tips, filtered, low retention, sterilized, 1000 μl |
One of the following suppliers:
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|
Pipette tips, wide bore, 200 μl |
Customer provided |
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|
Saline sodium citrate (SSC) (20X) |
Sigma-Aldrich, catalog # S6639 |
Equipment
|
Equipment |
Supplier |
|---|---|
|
Benchtop microcentrifuge 500 x g, suitable for 1.5 ml tubes and 15 ml conical tubes |
General lab supplier |
|
Hemocytometer or automated cell counter |
General lab supplier |
|
Ice bucket or cold blocks, suitable for 15 ml conical tubes and 1.5 ml microcentrifuge tubes |
General lab supplier |
|
Micropipettes, 1 µl–1000 µl capabilities |
General lab supplier |
