Consumables & Equipment
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The protocol requires the following user-supplied consumables and equipment.
Consumables
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Consumable |
Supplier |
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|---|---|---|---|---|---|---|---|---|---|---|
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1 M Tris-HCl pH 7.5 |
Teknova, catalog # T5075 |
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10% Bovine Serum Albumin (BSA) |
Sigma-Aldrich, catalog # A1595 |
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100% Anhydrous DMSO |
Thermo Fisher, catalog # D12345 |
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Absolute methanol (MeOH) |
Sigma-Aldrich, catalog # 34860 |
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Cell counting materials (eg, AO/PI) |
General lab supplier |
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Cell Suspension Buffer (CSB) |
Included in all Illumina Single Cell Prep kits |
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Conical tube, 15 ml, sterile and nuclease-free |
General lab supplier |
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DEPC-Treated Water (μl) |
Thermo Fisher, CAT#AM9906 |
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DTT (1 M) |
Sigma-Aldrich, catalog # 646563 |
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[Optional] DTT powder, solid |
Sigma-Aldrich, catalog # D9779, or equivalent |
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Low retention microcentrifuge tube, 1.5 ml |
General lab supplier |
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Nuclease-free water |
Thermo Fisher, catalog # AM9937, or equivalent |
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NxGen RNase inhibitor (40 U/μl) |
Biosearch Technologies, catalog # 30281-1 |
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Pierce DSP, No-Weigh Format |
Thermo Fisher, catalog # A35393 |
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Pipette tips, filtered, low retention, sterilized, 20 μl |
One of the following suppliers:
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Pipette tips, filtered, low retention, sterilized, 200 μl |
One of the following suppliers:
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Pipette tips, filtered, low retention, sterilized, 1000 μl |
One of the following suppliers:
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Pipette tips, wide-orifice, 200 μl |
Rainin, catalog # 30389241 |
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Saline sodium citrate (SSC) (20X) |
Sigma-Aldrich, catalog # S6639 |
Equipment
|
Equipment |
Supplier |
|---|---|
|
Benchtop microcentrifuge 500 × g, suitable for 1.5 ml tubes and 15 ml conical tubes |
General lab supplier |
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Hemocytometer or automated cell counter |
General lab supplier |
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Ice bucket or cold blocks, suitable for 15 ml conical tubes and 1.5 ml microcentrifuge tubes |
General lab supplier |
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Micropipettes, 1 µl–1000 µl capabilities (Rainin LTS-tip compatible pipettes are required if you are using the recommended Rainin pipette tips) |
General lab supplier |
