User-Supplied Consumables & Equipment

0.2 ml PCR 8-tube strips and caps or PCR reaction plates (if you have a compatible magnetic bead rack)

1.5 ml PCR clean centrifuge tubes

Absolute ethanol, molecular biology grade

Nuclease-free 1X low EDTA TE Buffer (IDTE), pH 8.0, 10 mM Tris, 0.1 mM EDTA

Nuclease-free water

Pipette tips, filtered, low retention, sterilized, 20 μl

Pipette tips, filtered, low retention, sterilized, 200 μl

Pipette tips, filtered, low retention, sterilized, 1000 μl

Oligonucleotide additive primers and associated index primers (Refer to Oligonucleotides)

Quantification consumables, any of the following:

Qubit 1X dsDNA High Sensitivity Assay Kit

0.2 ml/1.5 ml Magnetic Separation Combo Rack

Benchtop mini microcentrifuge 2000 × g, suitable for 1.5 ml tubes, 0.5 ml tubes and PCR tube strips

Micropipettes, 1–1000 µl capabilities

Quantification system, either of the following:

Qubit Fluorometer

Thermal cycler