Check Libraries
This step checks the concentration and quality of the final libraries.
| 1. | Quantify 2 μl of each sample using a Qubit High Sensitivity kit. |
| 2. | Assess average fragment size using an Agilent Bioanalyzer or Tapestation. |
| • | If necessary, dilute samples to make sure that they are within the appropriate range for the device. |
| • | Load an appropriate volume of the purified library on the fragment analyzer. |
Recommended loading concentration is 1–10 ng/µl on Tapestation or 1–2 ng/µl on Bioanalyzer.
Representative SNT library trace (expected avg. size 200–400 bp) for a 10,000 cell capture PBMC cell line on a High Sensitivity D1000 ScreenTape.
