Protocol

This section provides step-by-step instructions on how to use the SEA Kit to generate sequencing-ready enriched libraries using templated beads processed through a compatible Illumina Single Cell 3′ RNA workflow. Safe stopping points are indicated at the end of applicable steps.

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Before proceeding, confirm the kit contents and make sure that you have the necessary consumables and equipment.

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Follow the protocol in the order described using the specified parameters. Unless a safe stopping point is specified, move immediately to the next step.

Revision History

Document # 200064130 v00

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Illumina Single Cell RNA T100 Synthetic Nucleotide Tag Enrichment

t100-sea-snt