Prepare for Library Prep Protocol

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The following steps are performed in the pre-amplification area.

1. Remove the LP plate from storage and thaw on ice.
2. Remove the reagents from the box and prepare as follows.
Room Temperature Storage

Reagent

Box Name

Instructions

Protocol Step

IPB

Illumina Purification Beads, Pre-PCR

Use at room temperature.

Clean Up Ligation
-25°C to -15°C Storage

Reagent

Box Name

Instructions

Protocol Step

ATL4

Library Prep Box, Pre-PCR

Thaw at room temperature.

Perform A-Tailing

EPM4

Library Prep Box, Pre-PCR

Thaw on ice.

Index PCR

ERP6

Library Prep Box, Pre-PCR

Thaw on ice.

Prepare FFPE DNA and Perform End Repair.

LIGX

Library Prep Box, Pre-PCR

Thaw on ice.

Ligate Adapters

RSB

Library Prep Box, Pre-PCR

Bring to room temperature.

Prepare FFPE DNA, Perform End Repair, and Index PCR.

UD Index Plate

none

Thaw at room temperature.

Index PCR

UMI DIA

none

Thaw at room temperature.

Ligate Adapters