Product Contents

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Completing the Illumina FFPE DNA Prep with Exome 2.5 Enrichment protocol requires library prep and enrichment reagents, the Twist Bioscience for Illumina Exome 2.5 Panel, UMI DNA index anchors (UMI DIA), and index adapters.

Component

Kit Options

Catalog #

Library prep and enrichment reagents

Illumina Cell-Free and FFPE DNA Prep with Enrichment, Ligation (192 Samples, 4-Plex), Cloud Analysis

20104103

Illumina Cell-Free and FFPE DNA Prep with Enrichment, Ligation (192 Samples, 4-Plex), On-premises

20104104

Library prep only reagents

Illumina Cell-Free and FFPE DNA Prep, Ligation (16 Samples)

20104105

Illumina Cell-Free and FFPE DNA Prep, Ligation (96 Samples)

20104106

Enrichment only reagents

Illumina Cell-Free and FFPE DNA Prep, Enrichment (16 Reactions)

20104107

Enrichment probe panel

Twist Bioscience for Illumina Exome 2.5 Panel

20076914

[Optional] Supplemental Probe Panel

Twist Bioscience for Illumina Mitochondrial Panel

20093180

Illumina Custom Enrichment Panel v2 designed via DesignStudio (32 μl, 120bp)

20073953

Illumina Custom Enrichment Panel v2 designed via DesignStudio (384 μl, 120bp)

20073952

Illumina Custom Enrichment Panel v2 designed via DesignStudio (1536 μl, 120bp)

20111339

Index Adapters

IDT for Illumina UMI DNA/RNA UD Indexes Set A, Ligation (96 Indexes, 96 Samples)

20034701

IDT for Illumina UMI DNA/RNA UD Indexes Set B, Ligation (96 Indexes, 96 Samples)

20034702

IDT for Illumina UMI DNA/RNA UD Indexes Set A for Automation (96 Indexes, 96 Samples)

20066404

IDT for Illumina UMI DNA/RNA UD Indexes Set B for Automation (96 Indexes, 96 Samples)

20063213

Illumina UMI DNA/RNA UD v3 indexes Set A, Ligation (96 indexes, 96 Samples)

20126235

Illumina UMI DNA/RNA UD v3 indexes Set B, Ligation (96 indexes, 96 Samples)

20126237
Illumina DNA Prep, Ligation—Library Prep Box, Pre-PCR, Store at -25°C to -15°C

Tube Quantity

Reagent

Description

16 Samples

96 Samples

192 Samples

1

6

12

ATL4

A-Tailing Mix 4

1

4

8

EPM4

Enhanced PCR Mix 4

1

6

12

ERP6

End Repair Mix 6

1

4

8

LIGX

DNA Ligase X

1

1

2

RSB

Resuspension Buffer

Illumina Purification Beads, Pre-PCR, Store at Room Temperature

Tube Quantity

Reagent

Description

16 Samples

96 Samples

192 Samples

1

3

6

IPB

Illumina Purification Beads

Illumina DNA Prep, Ligation—Enrichment Box 1, Post-PCR, Store at -25°C to -15°C

Tube Quantity

Reagent

Description

16 Reactions

192 Samples

1

3

EE1

Enrichment Elution Buffer 1

1

3

EEW

Enhanced Enrichment Wash

1

3

EPM4

Enhanced PCR Mix 4

1

3

HP3

2 N NaOH

1

3

LNA1

Library Normalization Additives

1

3

NHB2

Hyb Buffer 2 + IDT NXT Blockers

2

6

PPC3

PCR Primer Cocktail 3

1

3

RSB

Resuspension Buffer

Illumina DNA Prep, Ligation—Enrichment Box 2, Post-PCR

Tube Quantity

Reagent

Description

Storage Temperature

16 Reactions

192 Samples

1

3

EHB2

Enrich Hyb Buffer 2

2°C to 8°C

1

3

IPB

Illumina Purification Beads

Room temperature

1

3

LNB1

Library Normalization Beads

2°C to 8°C

1

3

LNS1

Library Normalization Storage Buffer 1

2°C to 8°C

2

6

LNW1

Library Normalization Wash 1

2°C to 8°C

2

6

SMB3

Streptavidin Magnetic Beads 3

2°C to 8°C

For index adapter sequences, refer to Illumina Adapter Sequences.

Each kit contains one plate of IDT for Illumina UMI DNA Index Anchors and one plate of IDT for Illumina DNA/RNA UD Indexes Set A or B. For Illumina UMI DNA/RNA UD v3 indexes, each kit contains one plate of IDT for Illumina UMI DNA Index Anchors and one plate of Illumina DNA/RNA UD v3 indexes Set A or B.

Store at -25°C to -15°C

Description

IDT for Illumina UMI DNA/RNA UD Indexes Set A, Ligation (96 indexes, 96 samples) (UDP0001–UDP0096)

IDT for Illumina UMI DNA/RNA UD Indexes Set B, Ligation (96 indexes, 96 samples) (UDP0097–UDP0192)

IDT for Illumina UMI DNA/RNA UD Indexes Set A, Automation (96 indexes, 96 samples) (UDP0001–UDP0096)

IDT for Illumina UMI DNA/RNA UD Indexes Set B, Automation (96 indexes, 96 samples) (UDP0097–UDP0192)

Illumina UMI DNA/RNA UD v3 indexes Set A, Ligation (96 indexes, 96 Samples) (UDP0001–UDP0096)

Illumina UMI DNA/RNA UD v3 indexes Set B, Ligation (96 indexes, 96 Samples) (UDP0097–UDP0192)