Prepare for Protocol

1. Remove the reagents from the box and thaw as follows.
Room Temperature Storage

Reagent

Box Name

Instructions

IPB

Box 1

Use at room temperature.

ST2

Box 1

Use at room temperature.

2°C to 8°C Storage

Reagent

Box Name

Instructions

EBLTS

Box 2

Bring to room temperature.

TWB

Box 2

Bring to room temperature.

-25°C to -15°C Storage

Reagent

Box Name

Instructions

EPM

Box 3

Thaw on ice.

Index adapters

Illumina Unique Dual Indexes, LT

Thaw on ice.

RSB

Box 3

Thaw on ice.

TB1

Box 3

Thaw on ice.