Quantify and Normalize Libraries
1. | Analyze 2 µl library pool using a Qubit dsDNA HS Assay kit. |
If libraries are outside the standard range, dilute to 1:10 concentration and analyze again.
2. | Calculate the molarity value using the following formula. |
• | Use |
3. | Dilute each library pool to a minimum of 30 µl at a normalized concentration 4 nM using RSB. |