Quantify and Normalize Libraries

1. Analyze 2 µl library pool using a Qubit dsDNA HS Assay kit.

If libraries are outside the standard range, dilute to 1:10 concentration and analyze again.

2. Calculate the molarity value using the following formula.
Use 500 bp as the average library size if unable to analyze the library size using the BioanalyzerBioalnalyzer.

3. Dilute each library pool to a minimum of 30 µl at a normalized concentration 4 nM using RSB.