Quantify and Normalize Libraries

1.

Analyze 2 µl library pool using a Qubit dsDNA HS Assay kit.

If libraries are outside the standard range, dilute to 1:10 concentration and analyze again.

2.

Calculate the molarity value using the following formula.

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Use 500 bp as the average library size if unable to analyze the library size using the BioanalyzerBioalnalyzer.

3.

Dilute each library pool to a minimum of 30 µl at a normalized concentration 4 nM using RSB.