Overview

This resource explains how to prepare up to 48 uniquely indexed, targeted libraries from total RNA and/or DNA inputs using the Illumina Microbial Amplicon Prep workflow. This kit enables targeted sequencing (whole genome or select regions) of viruses and other microbes. Design your amplicon primer sets for your target(s) of interest and use those primer sets with the kit. The result is a targeted library from RNA and/or DNA for sequencing on Illumina systems. Results may vary depending on the targets and are not guaranteed.

Illumina Microbial Amplicon Prep (IMAP) offers:

•

Preparation of ≤ 48 unique dual-indexed libraries

•

Scalable library prep from 1–48 samples

•

Flexible nucleic acid inputs, RNA or DNA, depending on the nature of the target(s)

•

Compatibility with user-designed target primer sets

•

Generation of sequence-ready libraries in < 9 hours

After the targeted libraries are sequenced, data analysis is performed using the DRAGEN Targeted Microbial App available on BaseSpace Sequence Hub. This application aligns reads to one or more appropriate reference genomes, and then calls sequence variants. The application then applies those variants to the reference genome to generate one or more consensus sequences representing the population of nucleic acid species in the sample. When available, additional lineage analysis is performed from curated databases such as NextClade and Pangolin.