Product Contents

Completing the Illumina Microbial Amplicon Prep protocol requires one of the following library prep reagents kits that applies to your sequencing system, and the index adapters.

Component	Library Prep Kit Options	Catalog #
Library prep reagents	iSeq 100 System: iSeq 100 i1 Reagent v2 (300 Cycles)	20031371
	MiSeq System v2 reagent kit: MiSeq Reagent Kit v2 (300 Cycles)	MS-102-2002
	MiSeq System v3 reagent kit: MiSeq Reagent Kit v3 (600 Cycles)	MS-102-3003
	MiniSeq System: MiniSeq High Output Reagent Kit (300 Cycles)	FC-420-1003
Index adapters	Illumina Unique Dual Indexes, LT	20098166

Reagent Kit Contents

Each library prep reagent kit comprises four boxes. The following tables list the contents of each box. Promptly store reagents at the indicated temperature to ensure proper performance.

Illumina Microbial Amplicon Prep Box 1, Store at Room Temperature

Box 1—48 Samples, Component Box # 20093449
Quantity	Reagent	Description
1	IPB	Illumina Purification Beads
1	ST2	Stop Tagment Buffer 2

Illumina Microbial Amplicon Prep Box 2, Store at 2°C to 8°C

Box 2 – 48 Samples, Component Box # 20093450
Quantity	Reagent	Description
1	EBLTS	Enrichment BLT
1	TWB	Tagmentation Wash Buffer

Illumina Microbial Amplicon Prep Box 3, Store at -25°C to -15°C

Box 3 – 48 Samples, Component Box # 20093451
Quantity	Reagent	Description
2	EPH3	Elution Prime Fragment 3HC Mix
2	EPM	Enhanced PCR Mix
2	FSM	First Strand Mix
2	IPM	Illumina PCR Mix
2	RSB	Resuspension Buffer
1	RVT	Reverse Transcriptase
3	TB1	Tagmentation Buffer 1

Illumina Microbial Amplicon Prep Box 4, Store at -25°C to -15°C

Box 4 – 48 Samples, Indexes #20098166
Quantity	Description
1	Illumina Unique Dual Indexes, LT