Blood and Saliva Input Recommendations
The Illumina DNA Prep with Exome 2.5 Enrichment protocol is compatible with fresh whole blood (requires the Flex Lysis Reagent Kit) and saliva sample inputs. For information about protocols specific to blood and saliva, refer to Blood Lysis (Optional) or Saliva Lysis (Optional).
Blood and saliva protocols have not been tested in an enrichment workflow that combines the Twist Bioscience for Illumina Exome 2.5 Panel and Mitochondrial Panel.
When starting with 10 µl whole blood in EDTA, or 30 µl saliva in Oragene, expect normalization of pre-enrichment libraries equal to 50–1000 ng gDNA input. Blood and saliva are heterogeneous sample types. The Illumina DNA Prep with Exome 2.5 Enrichment kits ability to generate normalized libraries depends on the total amount of DNA obtained from the lysed sample. The following factors can adversely affect normalization of library independent of kit performance:
|
•
|
Viscosity of the saliva samples |
|
•
|
Sample storage conditions |
|
•
|
Underlying medical conditions affecting white blood cell counts |
