Check Exome-Enriched Library Pools

Perform the following to check the concentration and quality of each exome-enriched library pool.

1.

Run 1 µl enriched libraries using the Qubit dsDNA BR Assay Kit to quantify library concentration.

2.

Run 1 µl pooled library or the individual libraries on the Agilent Technology 2100 Bioanalyzer using a High Sensitivity DNA kit.

Expect a mean fragment size ~350 bp and distribution of DNA fragments with a size range from ~200 bp to ~1000 bp.