Product Contents

Completing the Illumina DNA Prep with Exome 2.5 Enrichment protocol requires library prep and enrichment reagents, the exome panel, clean up/size selection beads, and index adapters. All are included in the full kits listed. The number of index adapters required depends on the number of samples to be uniquely indexed for your experiment. Depending on the sample input type and sequencing requirements, the protocol might require additional, optional consumables.

Component

Kit Options

Illumina Catalog #

Library prep and enrichment reagents

Illumina DNA Prep with Exome 2.5 Enrichment Kit, Tagmentation Set B (96 Samples, 12-plex)

20077595

Illumina DNA Prep with Exome 2.5 Enrichment Kit, Tagmentation Set D (96 Samples, 12-plex)

20077596

[Optional] Supplemental Probe Panels

Twist Bioscience for Illumina Mitochondrial Panel (96 Samples, 12-plex)

20093180

Illumina Custom Enrichment Panel v2 via DesignStudio (32 μl, 120 bp)

20073953

Illumina Custom Enrichment Panel v2 via DesignStudio (384 μl, 120 bp)

20073952

Illumina Custom Enrichment Panel v2 via DesignStudio (1536 μl, 120 bp)

20111339

[Optional] Index adapters

Illumina DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20091654

Illumina DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)

20091656

Illumina DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)

20091658

Illumina DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples)

20091660

[Optional] Blood lysis¹

Flex Lysis Reagent Kit (96 samples)

20018706

¹ Required when starting the protocol from fresh whole blood samples and not purified blood gDNA.

Illumina DNA/RNA Prep - IPB Tagmentation Buffers, Store at 15°C to 30°C*

Tube Quantity

(96 Samples)

Acronym

Reagent Name

Tube Cap Color

4

ST2

Stop Tagment Buffer 2

Red

1

TWB

Tagmentation Wash Buffer

Clear

2

IPB

Illumina Purification Beads

Red

* The reagents are shipped at 2°C to 8°C. Promptly store reagents at the indicated temperature to ensure proper performance.

Illumina DNA Prep - Tagmentation (S) Beads, Store at 2°C to 8°C

Tube Quantity

(96 Samples)

Acronym

Reagent Name

Tube Cap Color

4

eBLT*

Enrichment Bead-Linked Transposomes

Yellow

2

RSB

Resuspension Buffer

Clear

* Store the eBLT stock tube upright so that the beads are always submerged in the buffer.

Illumina DNA/RNA Prep - Tagmentation PCR Reagents, Store at ‑25°C to ‑15°C*

Tube Quantity

(96 Samples)

Acronym

Reagent Name

Tube Cap Color

4

TB1

Tagmentation Buffer 1

Clear

4

EPM

Enhanced PCR Mix

Clear

* The reagents are shipped at 2°C to 8°C. Promptly store reagents at the indicated temperature to ensure proper performance.

Twist BioScience for Illumina Exome 2.5 Panel, Store at ‑25°C to ‑15°C

Tube Quantity

(96 Samples)

Acronym

Reagent Name

Tube Cap Color

1

Not Applicable

Twist BioScience for Illumina Exome 2.5 Panel

Green
Illumina DNA Fast Hyb - Enrichment Beads + Buffers, Store at 2°C to 8°C

Tube Quantity

(96 Samples)

Acronym

Reagent Name

Tube Cap Color

2

SMB3

Streptavidin Magnetic Beads

Clear

1

RSB

Resuspension Buffer

Clear

1

EHB2

Enrich Hyb Buffer 2

Clear

1

ET2

Elute Target Buffer 2

Clear
Illumina DNA Fast Hyb - Enrichment PCR + Buffers, Store at ‑25°C to ‑15°C*

Tube Quantity

(96 Samples)

Acronym

Reagent Name

Tube Cap Color

1

EE1

Enrichment Elution Buffer 1

Clear

4

EEW

Enhanced Enrichment Wash

Amber

1

PPC

PCR Primer Cocktail

Clear

1

HP3

2 N NaOH

Clear

1

NHB2

Hyb Buffer 2 + IDT NXT Blockers

Blue

1

EPM

Enhanced PCR Mix

Clear

* The reagents are shipped at 2°C to 8°C. Promptly store reagents at the indicated temperature to ensure proper performance.

For index adapter sequences, refer to Illumina Adapter Sequences.

The index set received depends on the kit ordered with one containing Set B and the other Set D.

Illumina DNA/RNA UD Indexes, Store at ‑25°C to ‑15°C

Description

Illumina DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)

Illumina DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples)

[Optional] Illumina DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

[Optional] Illumina DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)
Store at ‑25°C to ‑15°C

Description

Twist Bioscience for Illumina Mitochondrial Panel (96 samples, 12-plex)
Store at ‑25°C to ‑15°C

Description

Illumina Custom Enrichment Panel v2 (32 μl, 120 bp)

Illumina Custom Enrichment Panel v2 (384 μl, 120 bp)

Illumina Custom Enrichment Panel v2 (1536 μl, 120 bp)

The following reagents are shipped at -25°C to -15°C. Promptly store reagents at the indicated storage temperature to ensure proper performance.

Quantity

Acronym

Reagent Name

Tube Cap Color

Storage Temperature

4

BLB

Blood Lysis Buffer

Clear

15°C to 30°C

4

PK1

Proteinase K

Clear

-25°C to -15°C