Protocol
This section describes the Illumina DNA Prep with Exome 2.5 Enrichment protocol, with optional Mitochondrial DNA and/or custom target enrichment.
| • | Review the planned complete sequencing workflow, from sample through analysis, to ensure compatibility of products and experiment parameters. |
| • | Before proceeding, confirm kit contents and make sure that you have the required components, equipment, and consumables. This protocol includes the library prep and enrichment reagents, Twist BioScience for Illumina Exome 2.5 Panel, IPB, and index adapter plates. Refer to Consumables & Equipment. |
| • | Follow the protocol in the order shown, using the specified volumes and incubation parameters. |
Illumina DNA Prep with Exome 2.5 Enrichment Workflow
The following diagram illustrates the Illumina DNA Prep with Exome 2.5 Enrichment workflow. Safe stopping points are marked between steps.
Time estimates are based on processing 12 samples with a single, 12-plex enrichment reaction.
