Blood and Saliva Input Recommendations
The Illumina DNA Prep protocol is compatible with fresh whole blood (requires the Flex Lysis Reagent Kit), dried blood (requires BLT, and saliva sample inputs. For information about protocols specific to blood and saliva, refer to Blood Lysis (Optional), Dried Blood Spot Extraction (Optional), or Saliva Lysis (Optional).
When starting with 10 µl liquid whole blood in EDTA tubes or 30 µl saliva in Oragene tubes, expect normalization of libraries equal to results observed when using ≥ 100 ng DNA input. Blood and saliva are heterogeneous sample types. Therefore the ability of Illumina DNA Prep to generate normalized libraries depends on the total amount of DNA obtained from the lysed sample. The following factors can adversely affect normalization of library independent of kit performance:
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Viscosity of the saliva samples |
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Sample storage conditions |
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Underlying medical conditions affecting white blood cell counts |
