Overview

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This guide explains how to prepare up to 384 unique dual-indexed paired-end libraries from DNA using the Illumina DNA Prep workflow.

The Illumina DNA Prep workflow:

• Uses tagmentation, an enzymatic reaction, to fragment DNA and add adapter sequences in only 15 minutes.
• Innovates sample normalization at inputs ≥ 100 ng.
• Streamlines sample pooling and sequencing.
• Master mixed reagents reduce containers, pipetting, and hands-on time.
• Requires as little as 1 ng input.
• Can prepare libraries directly from whole blood or saliva samples when using an extraction protocol.

The Illumina DNA Prep library prep kit uses a bead-based transposome complex to tagment genomic DNA, which is a process that fragments DNA and then tags the DNA with adapter sequences in one step. The bead-based transposome complex then fragments a set number of DNA molecules. This fragmentation provides flexibility to use a wide DNA input range to generate normalized libraries of consistent tight fragment size distribution. Following tagmentation, a limited-cycle PCR adds adapter sequences to the ends of a DNA fragment. This step enables compatibility across all Illumina sequencing systems. A subsequent Illumina Purification Beads (IPB) cleanup step then purifies libraries for use on an Illumina sequencing system.