Product Contents
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Completing the Illumina DNA Prep protocol requires library prep reagents and index adapters. The number of index adapters required depends on the number of samples to be uniquely indexed for your experiment.
Depending on the sample input type and sequencing requirements, the protocol might require additional, optional consumables.
|
Component |
Kit Options |
Catalog # |
|---|---|---|
|
Library prep reagents |
Illumina DNA Prep (24 Samples) |
20060060 |
|
Illumina DNA Prep (96 Samples) |
20060059 |
|
|
Index adapters¹ |
||
|
Illumina DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples) |
20091654 |
|
|
Illumina DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples) |
20091656 |
|
|
Illumina DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples) |
20091658 |
|
|
Illumina DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples) |
20091660 |
|
|
IDT for Illumina - DNA/RNA UD Set A, Tagmentation (96 Indexes, 96 Samples) (Obsolete) |
20027213 |
|
|
IDT for Illumina - DNA/RNA UD Set B, Tagmentation (96 Indexes, 96 Samples) (Obsolete) |
20027214 |
|
|
IDT for Illumina - DNA/RNA UD Set C, Tagmentation (96 Indexes, 96 Samples) (Obsolete) |
20042666 |
|
|
IDT for Illumina - DNA/RNA UD Set D, Tagmentation (96 Indexes, 96 Samples) (Obsolete) |
20042667 |
|
|
Nextera DNA CD Indexes (96 Indexes, 96 Samples) |
20018708 |
|
|
[Optional] Blood lysis² |
Flex Lysis Reagent Kit (96 samples) |
20018706 |
¹ Index kits are not interchangeable with Nextera XT index kits.
² Required when starting the protocol from fresh whole blood samples.
Library Prep Kit Contents
Illumina DNA Prep Beads and Buffers, Store at room temperature*
|
Tube Quantity |
Acronym |
Reagent Name |
Storage Temperature |
|
|---|---|---|---|---|
|
24 Samples |
96 Samples |
|||
|
1 |
2 |
IPB |
Illumina Purification Beads |
Room temperature |
|
1 |
4 |
TSB |
Tagment Stop Buffer |
Room temperature |
|
1 |
1 |
TWB |
Tagment Wash Buffer |
Room temperature |
* These reagents are shipped at 2°C to 8°C. Promptly store reagents at the indicated tube temperature to ensure proper performance.
Illumina DNA Prep PCR and Buffers, Store at -25°C to -15°C
|
Tube Quantity |
Acronym |
Reagent Name |
|
|---|---|---|---|
|
24 Samples |
96 Samples |
||
|
1 |
1 |
RSB |
Resuspension Buffer |
|
1 |
4 |
TB1 |
Tagmentation Buffer 1 |
|
1 |
4 |
EPM |
Enhanced PCR Mix |
Illumina DNA Prep Tagmentation (M) Beads, Store at 2°C to 8°C
|
Tube Quantity |
Acronym |
Reagent Name |
|
|---|---|---|---|
|
24 Samples |
96 Samples |
||
|
1 |
4 |
BLT |
Bead-Linked Transposomes |
Index Kit Contents
For index adapter sequences, refer to Illumina Adapter Sequences.
IDT for Illumina DNA/RNA UD Indexes, IDT for Illumina Nextera DNA UD Indexes, or Nextera DNA CD Indexes (96 Indexes, 96 Samples) Plates, Store at -25°C to -15°C
|
Quantity |
Description |
|---|---|
|
1 |
96 Dual Adapter Index Plate |
[Optional] Flex Lysis Reagent Kit* (Catalog #20018706)
|
Quantity |
Acronym |
Description |
Storage Temperature |
|---|---|---|---|
|
4 |
BLB |
Blood Lysis Buffer |
Room Temperature |
|
4 |
PK1 |
Proteinase K |
-25°C to -15°C |
* These reagents are shipped at -25°C to -15°C. Promptly store reagents at the indicated tube temperature to ensure proper performance.
Illumina Purification Beads are included in the Illumina DNA Prep 24 sample and 96 sample kits.
[Optional] Illumina Purification Beads
|
Acronym |
Description |
Catalog # |
|---|---|---|
|
IPB |
30 ml |
20119944 |
|
IPB |
100 ml |
20060057 |
|
IPB |
400 ml |
20060058 |
