Protocol

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This section describes the Illumina DNA Prep protocol.

• Review the planned complete sequencing workflow, from sample through analysis, to ensure compatibility of products and experiment parameters.
• Before proceeding, confirm kit contents and make sure that you have the required components, equipment, and consumables. This protocol requires library prep reagents and index adapters. Index adapters are sold separately. Refer to Consumables & Equipment.
• Follow the protocol in the order shown, using the specified volumes and incubation parameters.

If you plan to pool libraries, record information about your samples before starting library prep using Illumina Experiment Manager (IEM), Local Run Manager, or BaseSpace Sequence Hub. For information on the tools compatible with your sequencing system, visit the Illumina DNA Prep Compatible Products support page.

• For low-plexity pooling strategies (2-plex to 9-plex), refer to the Index Adapters Pooling Guide.
• For index adapter sequences and information about recording the sequences, refer to Illumina Adapter Sequences.

The following diagram illustrates the Illumina DNA Prep workflow.

• Safe stopping points are marked between steps.
• Time estimates are based on preparing 16 samples using a multichannel pipette.

Illumina DNA Prep Workflow