Overview

The Illumina RNA Prep with Enrichment, (L) Tagmentation kit pairs with an index kit and enrichment panel to generate enriched libraries for dual-indexed, paired-end sequencing. Reverse transcription converts RNA into complementary DNA (cDNA), which is then tagmented and amplified to add indexes and other adapters. The resulting libraries are normalized for one- or three-plex enrichment and further amplification.

The Illumina RNA Prep with Enrichment, (L) Tagmentation 16 sample kit (20040536) includes reagents for preparing and enriching 16 libraries (16, 1-plex enrichment reactions). The Illumina RNA Prep with Enrichment, (L) Tagmentation96 sample kit (20040537) includes reagents for preparing and enriching 96 libraries (32, 3-plex enrichment reactions). Enrichment probe panel, purification beads and index adapters must be purchased separately.

Sequence-specific biotinylated probes combine with magnetic beads to capture regions of interest. The captured sequences are washed, eluted, and amplified to generate copies of the enriched library. A limited-cycle PCR program exponentially amplifies the enriched fragments, copying each fragment to increase the amount of library.

The kit offers the following features:

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Tagmentation with Enrichment Bead-Linked Transposomes (EBLTL) to create larger inserts

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Unique dual (UD) indexing with the Illumina DNA/RNA UD Indexes

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High-quality sequencing data from a range of input, 10–100 ng total RNA