Product Contents
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Make sure that you have all reagents identified in this section before starting library prep. The protocol requires one Illumina RNA Prep with Enrichment, (L) Tagmentation kit, one panel, and at least one Illumina DNA/RNA UD Indexes set. Combine all four index adapter sets to index 384 libraries.
| • | The required library preparation kits provide reagents for denaturing, cDNA synthesis, library prep, and enrichment in a 16-sample 1-plex workflow, or 96-sample 3-plex workflow. |
| • | The enrichment panels provide application-specific oligos. |
| • | The index sets provide premixed Index 1 (i7) and Index 2 (i5) adapters. |
Illumina Purification Beads (IPB) are also required. These reagents are not included in the Illumina kits and must be purchased separately. Refer to User-Supplied Consumables and Equipment for the IPB quantity requirements and catalog numbers.
|
Component |
Name |
Catalog # |
|---|---|---|
|
Library prep reagents |
Illumina RNA Prep with Enrichment, (L) Tagmentation (16 Samples)¹ |
20040536 |
|
Illumina RNA Prep with Enrichment, (L) Tagmentation (96 Samples)² |
20040537 |
|
|
Index adapters |
Illumina DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples) |
20091654 |
|
Illumina DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples) |
20091656 |
|
|
Illumina DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples) |
20091658 |
|
|
Illumina DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples) |
20091660 |
|
|
IDT for Illumina DNA/RNA UD Set A, Tagmentation (96 Indexes, 96 Samples) (Obsolete) |
20027213 |
|
|
IDT for Illumina DNA/RNA UD Set B, Tagmentation (96 Indexes, 96 Samples) (Obsolete) |
20027214 |
|
|
IDT for Illumina DNA/RNA UD Set C, Tagmentation (96 Indexes, 96 Samples) (Obsolete) |
20042666 |
|
|
IDT for Illumina DNA/RNA UD Set D, Tagmentation (96 Indexes, 96 Samples) (Obsolete) |
20042667 |
|
|
Enrichment panels |
Illumina Exome Panel |
20020183 |
|
Respiratory Virus Oligo Panel v2 |
20044311 |
|
|
Pan-Coronavirus Panel |
20088155 |
|
|
Respiratory Virus Enrichment Kit Set A (96 samples) |
20100469 |
|
|
Respiratory Virus Enrichment Kit Set B (96 samples) |
20100470 |
|
|
Respiratory Virus Enrichment Kit Set C (96 samples) |
20100471 |
|
|
Respiratory Virus Enrichment Kit Set D (96 samples) |
20100472 |
|
|
Illumina Viral Surveillance Panel, RUO 96 samples |
20088154 |
|
|
Illumina Viral Surveillance Panel Enrichment Kit Set A (96 samples) |
20087932 |
|
|
Illumina Viral Surveillance Panel Enrichment Kit Set B (96 samples) |
20087929 |
|
|
Respiratory Pathogen ID/AMR Enrichment kits |
Respiratory Pathogen ID/AMR Panel Enrichment Kit (RUO) A |
20047050 |
|
Respiratory Pathogen ID/AMR Panel Enrichment Kit (RUO) B |
20046969 |
|
|
Respiratory Pathogen ID/AMR Panel Enrichment Kit (RUO) C |
20047051 |
|
|
Respiratory Pathogen ID/AMR Panel Enrichment Kit (RUO) D |
20047052 |
¹Kit includes reagents for 16 one-plex enrichment reactions.
²Kit includes reagents for 32 three-plex enrichment reactions.
Reagent Kit Contents
Illumina cDNA Synthesis, Store at ‑25°C to ‑15°C
|
Tube Quantity |
Reagent |
Description |
|
|---|---|---|---|
|
16 Samples |
96 Samples |
||
|
1 |
4 |
EPH3 |
Elute, Prime, Fragment High Concentration Mix |
|
1 |
4 |
FSA |
First Strand Synthesis Act D Mix |
|
1 |
2 |
RSB |
Resuspension Buffer This RSB is stored frozen. |
|
1 |
1 |
RVT |
Reverse Transcriptase |
|
1 |
4 |
SMM |
Second Strand Marking Master Mix |
Illumina DNA/RNA Prep — Tagmentation PCR Reagents, Store at -25°C to -15°C*
|
Tube Quantity |
Reagent |
Description |
|
|---|---|---|---|
|
16 Samples |
96 Samples |
||
|
2 |
4 |
EPM |
Enhanced PCR Mix |
|
1 |
4 |
TB1 |
Tagmentation Buffer 1 |
*Shipped at 2°C to 8°C.
Illumina RNA Fast Hyb Enrichment PCR + Buffers, Store at -25°C to -15°C*
|
Tube Quantity |
Reagent |
Description |
|
|---|---|---|---|
|
16 Samples |
96 Samples |
||
|
1 |
2 |
EE1 |
Enrichment Elution Buffer 1 |
|
1 |
4 |
EEW |
Enhanced Enrichment Wash |
|
1 |
2 |
EPM |
Enhanced PCR Mix |
|
1 |
1 |
HP3 |
2 N NaOH |
|
1 |
1 |
NHB2 |
Hyb Buffer 2 + IDT NXT Blockers |
|
1 |
1 |
PPC |
PCR Primer Cocktail |
*Shipped at 2°C to 8°C.
Illumina DNA/RNA Prep — Tagmentation Beads, Store at 2°C to 8°C
|
Tube Quantity |
Reagent |
Description |
|
|---|---|---|---|
|
16 Samples |
96 Samples |
||
|
1 |
4 |
EBLTL |
Enrichment Bead-Linked Transposomes |
|
1 |
2 |
RSB |
Resuspension Buffer This RSB is stored cold. |
Illumina RNA Fast Hyb Enrichment Beads + Buffers, Store at 2°C to 8°C
|
Tube Quantity |
Reagent |
Description |
|
|---|---|---|---|
|
16 Samples |
96 Samples |
||
|
1 |
1 |
EHB2 |
Enrich Hyb Buffer 2 |
|
1 |
1 |
ET2 |
Elute Target Buffer 2 |
|
1 |
1 |
RSB |
Resuspension Buffer This RSB is stored cold. |
|
1 |
2 |
SMB4 |
Streptavidin Magnetic Beads 4 |
Illumina DNA/RNA Prep — Tagmentation Buffers, Store at Room Temperature*
|
Tube Quantity |
Reagent |
Description |
|
|---|---|---|---|
|
16 Samples |
96 Samples |
||
|
1 |
4 |
ST2 |
Stop Tagment Buffer 2 |
|
1 |
1 |
TWB |
Tagmentation Wash Buffer |
*Shipped at 2°C to 8°C.
Index Adapters and Enrichment Panels
Illumina DNA/RNA UD Indexes (96 Indexes, 96 Samples), Store at -25°C to -15°C
|
Quantity |
Reagent |
Description |
|---|---|---|
|
1 |
UDP0001–UDP0096 |
Set A index adapter plate |
|
1 |
UDP0097–UDP0192 |
Set B index adapter plate |
|
1 |
UDP0193–UDP0288 |
Set C index adapter plate |
|
1 |
UDP0289–UDP0384 |
Set D index adapter plate |
Enrichment Panels, Store at -25°C to -15°C
|
Panel Name |
Quantity |
Reagent |
Description |
|---|---|---|---|
|
Illumina Exome Panel |
1 |
CEX |
Coding Exome Oligos |
|
Pan-Coronavirus Panel |
1 |
Pan-CoV |
Pan-Coronavirus Oligos |
|
Viral Surveillance Panel |
1 |
VSP |
Viral Surveillance Panel Oligos |
|
Respiratory Virus Oligo Panel v2 |
1 |
RVO |
Respiratory Virus Oligos |
|
Respiratory Pathogen ID/AMR Panel |
1 |
RPIP |
Respiratory Pathogen ID/AMR Oligos |
