Protocol
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This section describes the Illumina RNA Prep with Enrichment, (L) Tagmentation protocol and provides instructions for preparing and enriching libraries.
| • | Review the complete sequencing workflow, from sample through analysis, to ensure compatibility of products and experiment parameters. |
| • | Confirm kit contents and make sure that you have the required consumables and equipment including library prep and enrichment reagents, an enrichment panel, and index adapters. For a complete list, refer to Consumables and Equipment. |
Pooling Preparation
When pooling libraries, record information about your samples before starting library prep. Use a recording tool compatible with your sequencing system and libraries. For compatibility information, refer to the Respiratory Pathogen ID/AMR Panel support pages or the support pages for your system.
The protocol uses Illumina DNA/RNA UD Indexes to index libraries. These
| • | For strategies on forming low-plex, color-balanced pools, refer to the Index Adapters Pooling Guide. |
| • | For index adapter sequences and how to record them, refer to Illumina Adapter Sequences. |
Illumina RNA Prep with Enrichment, (L) Tagmentation Workflow
The following diagram provides an overview of the Illumina RNA Prep with Enrichment protocol using a single sample. Safe stopping points are marked between steps.
