Protocol
This section describes the Illumina Stranded mRNA Prep, Ligation protocol with step-by-step instructions.
• | Review the complete sequencing workflow, from sample through analysis, to ensure compatibility of products and experiment parameters. |
• | Confirm kit contents and make sure that you have the required consumables and equipment, including library prep reagents and index anchors and adapters. For a complete list, refer to Consumables & Equipment. |

The following diagram provides an overview of the Illumina Stranded mRNA Prep protocol when using 24 samples. Safe stopping points are marked between steps.