Overview
The Illumina Stranded mRNA Prep, Ligation kit converts the messenger RNA (mRNA) in total RNA into up to 384 dual-indexed libraries.
Oligo(dT) magnetic beads purify and capture the mRNA molecules containing polyA tails. The purified mRNA is fragmented and copied into first strand complimentary DNA (cDNA) using reverse transcriptase and random primers. In a second strand cDNA synthesis step, dUTP replaces dTTP to achieve strand specificity. The final steps add adenine (A) and thymine (T) bases to fragment ends and ligate adapters. The resulting products are purified and selectively amplified for sequencing on an Illumina system.
Illumina Stranded mRNA Prep, Ligation offers the following features:
| • | A polyA capture to selectively sequence mRNA |
| • | Capture of both coding RNA and multiple forms of noncoding RNA that are polyadenylated |
| • | Unique dual (UD) indexing with the lllumina RNA UD Indexes |
