Revision History

Document

Date

Description of Change

Document # 1000000124518 v04

October 2023

Added Illumina RNA UD Indexes set A-D, Ligation to list of index adapters options.

Added recommendations for using the TapeStation System and example trace diagrams.

Added DynaMag-96 Side Magnet to equipment list as an option for the pipette workflow.

Corrected product contents for library prep kit to include RPBX.

Revised recommended guidance for quantifying total RNA input.

Added HTML format.

Document # 1000000124518 v03

June 2022

Added

• the term universal to anchor ligation.
• NextSeq 2000 custom recipe with a cycle 1 dark cycle.
• guidance for reusing samples in handling reagents.

Updated

• 16 sample kit configuration.
• reference to anchor plate color.
• title to include ligation.
• consumables and equipment to include Kapa qPCR lib quant kit and qPCR instrument for NextSeq 2000 users.
• bioanalyzer traces to include dimer peaks and DNase treatment must be done while running your preferred extraction method.
• Organized consumables in alphabetical order.
• grammar and format throughout to match style guide.
• check library steps updated for clarity.

Document # 1000000124518 v02

April
2021

Added dilution and quantitation information for the NextSeq 1000/2000 Sequencing System.

Removed Agencourt RNAClean XP Beads.

Corrected EPH3 and FSA definitions.

 

Document # 1000000124518 v01

August 2020

Updated workflow diagram description to include the number of samples used to calculate processing times.

Corrected formatting of index kit names.

 

Document # 1000000124518 v00

June 2020

Initial release.