User-Supplied Consumables & Equipment

1.7 ml microcentrifuge tubes, RNase-free

20 µl barrier pipette tips

200 µl barrier pipette tips

1000 µl barrier pipette tips

96-well twin.tec 250 µl PCR plates, semiskirted

Agencourt AMPure XP, 60 ml

Agilent DNA 1000 Kit

Conical centrifuge tubes (15 ml or 50 ml)

Ethyl alcohol, pure (500 ml)

Microseal 'B' PCR Plate Sealing Film

Nuclease-free ultrapure water

RNase/DNase-free multichannel reagent reservoirs, disposable

RNaseZap*

[Optional] Universal Human Reference RNA positive sample control

[Optional] Agilent RNA 6000 Pico Kit

[Optional] Qubit Assay Tubes

[Optional] Qubit dsDNA BR Assay Kit

[Optional] KAPA Library Quantification Kit

20 µl multichannel pipettes

20 µl single channel pipettes

200 µl multichannel pipettes

200 µl single channel pipettes

1000 µl single channel pipettes

One of the following analyzers:

[Shaking workflow] BioShake iQ high-speed thermoshaker

[Shaking workflow] BioShake PCR plate adapter

One of the following magnets:

[Shaking workflow]

[Pipette workflow]

Microplate centrifuge

One of the following 96-well thermal cyclers:

Vortexer