Prepare for Library Prep Protocol
The following steps are performed in the pre-amplification area.
| 1. | Remove the cfDNA from storage and thaw on ice. |
| 2. | Remove the reagents from the box and prepare as follows. |
|
Reagent |
Box Name |
Instructions |
Protocol Step |
|---|---|---|---|
|
IPB |
Illumina Purification Beads, Pre-PCR |
Use at room temperature. |
Clean Up Ligation |
|
Reagent |
Box Name |
Instructions |
Protocol Step |
|---|---|---|---|
|
ATL4 |
Library Prep Box, Pre-PCR |
Thaw at room temperature. |
Perform A-Tailing |
|
EPM4 |
Library Prep Box, Pre-PCR |
Thaw on ice. |
Index PCR |
|
ERP6 |
Library Prep Box, Pre-PCR |
Thaw on ice. |
Prepare cfDNA and Perform End Repair |
|
LIGX |
Library Prep Box, Pre-PCR |
Thaw on ice. |
Ligate Adapters |
|
RSB |
Library Prep Box, Pre-PCR |
Bring to room temperature. |
Prepare cfDNA and Perform End Repair, Index PCR |
|
UD Index Plate |
N/A |
Thaw at room temperature. |
Index PCR |
|
UMI DIA |
N/A |
Thaw at room temperature. |
Ligate Adapters |
