Post Tagmentation Clean Up

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This step washes the adapter-tagged DNA on the BLT-LR before amplification.

• Stop Tagment Buffer 2 (ST2)
• Tagmentation Wash Buffer 2 (TWB2)
1. Prepare the following consumables:
• ST2—If precipitates are observed, vortex until dissolved.
• TWB2—Invert to mix.
1. Remove from the thermal cycler and add 10 μl ST2 to each well.
2. Using a pipette set to 30 µl, pipette to mix.
3. Incubate at room temperature for 2 minutes.
4. Wash beads as follows.
a. Place on the magnetic stand and wait until the liquid is clear (~3 minutes).
b. Remove and discard all supernatant from each well.
c. Remove from the magnetic stand.
d. Add 100 µl TWB2 to each well.
e. Pipette to mix until beads are fully resuspended.
5. Wash beads a second time.

After the second wash, keep pellets in TWB2 to prevent overdrying of the beads.

6. Place on the magnetic stand and wait until the liquid is clear (~2 minutes).