Protocol

This section describes the Illumina Complete Long Read Prep with Enrichment protocol.

• Review the planned complete sequencing workflow, from sample through analysis, to ensure compatibility of products and experiment parameters.
• Before proceeding, confirm kit contents and make sure that you have the required components, equipment, and consumables. This protocol requires library prep reagents and index adapters. Index adapter plates are ordered separately. Refer to Product Contents for details.
• Follow the protocols in the order shown, using the specified volumes and incubation parameters.
• Before beginning library preparation, record sample information, and assign each sample unique dual indexes.

Make sure that the indexes used are unique for each library. Some indexes in the Set B and Set D Illumina DNA/RNA Unique Dual Indexes (96 Indexes, 96 Samples) plates are also used in the Illumina DNA/RNA Unique Dual Indexes (48 Indexes, 48 Samples) plate. Use of non-unique indexes can lead to failure in sample differentiation and FASTQ generation.

The following diagram illustrates the Illumina Complete Long Read Prep with Enrichment Kit workflow.

• Safe stopping points are marked between steps.
• Time estimates are based on preparing 12 samples using a multichannel pipette.