Illumina-Supplied Consumables

Completing the Nextera XT protocol requires library prep reagents and index adapters.

Component

Kit Options

Catalog #

Library prep reagents

Nextera XT DNA Library Preparation Kit

(24 Samples)

FC-131-1024

Nextera XT DNA Library Preparation Kit

(96 Samples)

FC-131-1096

Index adapters

IDT for Illumina DNA/RNA UD Indexes Set A, Tagmentation

(96 Indexes, 96 Samples)

20027213

IDT for Illumina DNA/RNA UD Indexes Set B, Tagmentation

(96 Indexes, 96 Samples)

20027214

IDT for Illumina DNA/RNA UD Indexes Set C, Tagmentation

(96 Indexes, 96 Samples)

20042666

IDT for Illumina DNA/RNA UD Indexes Set D, Tagmentation

(96 Indexes, 96 Samples)

20042667

IDT for Illumina Nextera DNA Unique Dual Indexes Set C

(96 Indexes, 96 Samples)

20027215

IDT for Illumina Nextera DNA Unique Dual Indexes Set D

(96 Indexes, 96 Samples)

20027216

Nextera XT Index Kit v2 Set A

(96 Indexes, 384 Samples)

FC-131-2001

Nextera XT Index Kit v2 Set B

(96 Indexes, 384 Samples)

FC-131-2002

Nextera XT Index Kit v2 Set C

(96 Indexes, 384 Samples)

FC-131-2003

Nextera XT Index Kit v2 Set D

(96 Indexes, 384 Samples)

FC-131-2004

Nextera XT Index Kit

(24 Indexes, 96 Samples)

FC-131-1001

Accessory Products for Nextera XT

Illumina Purification Bead, 100 ml

20060057

Illumina Purification Bead, 400 ml

20060058

Box 1 of 2

These reagents are shipped at -25°C to -15°C. Promptly store reagents at the indicated tube temperature to ensure proper performance.

Tube Quantity

Acronym

Reagent Name

Storage Temperature

24 Samples

96 Samples

1

1

ATM

Amplicon Tagment Mix

-25°C to -15°C

1

2

TD

Tagment DNA Buffer

-25°C to -15°C

1

1

NPM

Nextera PCR Master Mix

-25°C to -15°C

1

4

RSB

Resuspension Buffer

-25°C to -15°C

1

1

LNA1

Library Normalization Additives 1

-25°C to -15°C

1

2

LNW1

Library Normalization Wash 1

2°C to 8°C

Box 2 of 2

These reagents are shipped at -25°C to -15°C. Promptly store reagents at the indicated tube temperature to ensure proper performance.

Tube Quantity

Acronym

Reagent Name

Storage Temperature

24 Samples

96 Samples

1

1

NT

Neutralize Tagment Buffer

Room temperature

1

1

LNB1

Library Normalization Beads 1

2°C to 8°C

1

1

LNS1

Library Normalization Storage Buffer 1

Room temperature

For index adapter sequences, refer to Illumina Adapter Sequences.

IDT for Illumina Nextera DNA UD Indexes (96 Indexes, 96 Samples) Plate Format, Store at -25°C to -15°C

Quantity

Description

1

96 Dual Adapter Index Plate

Nextera XT Index Kit v2 (96 indexes, 384 Samples), Store at -25°C to -15°C

Set

Quantity

Description

A

8 tubes

Index Primers, S502, S503, S505–S508, S510, and S511

12 tubes

Index Primers, N701–N707, N710–N712, N714, and N715

B

8 tubes

Index Adapters: S502, S503, S505–S508, S510, and S511

12 tubes

Index Adapters: N716, N718–N724, and N726–N729

C

8 tubes

Index Adapters: S513, S515–S518, and S520–S522

12 tubes

Index Adapters: N701–N707, N710–N712, N714, and N715

D

8 tubes

Index Adapters: S513, S515–S518, and S520–S522

12 tubes

Index Adapters: N716, N718–N724, and N726–N729

Index Adapter Replacement Caps, Store at 15°C to 30°C

Quantity

Description

1 bag

i7 Index Tube Caps, Orange

1 bag

i5 Index Tube Caps, White

Nextera XT Index Kit (24 Indexes, 96 Samples), Store at -25°C to -15°C

Quantity

Description

4 tubes

Index Adapters: S502–S504 and S517

6 tubes

Index Adapters: N701–N706

Index Adapter Replacement Caps, Store at 15°C to 30°C

Quantity

Description

1 bag

i7 Index Tube Caps, Orange

1 bag

i5 Index Tube Caps, White

TruSeq Index Plate Fixture Kit, Store at Room Temperature (Optional)

Each TruSeq Index Plate Fixture Kit contains two fixtures to help arrange index primers before dispensing to a 96-well plate during library amplification. The fixture pairs with both the 24-sample kit and 96-sample kit.

Quantity

Description

2

TruSeq Index Plate Fixture